ELECTROPHORETIC HOMOGENEITY OF PREGNANT MARE SERUM GONADOTROPHIN

Electrophoretic Homogeneity of Pregnant Mare Serum Gonadotrophin

A highly purified and potent gonadotrophin in pregnant mare serum has been prepared. The preparation has been shown to be electrophoretically homogeneous in the Tiselius apparatus. The mobilities of the substance have been determined over a wide range of hydrogen ion concentrations. The isoelectric point lies at pH 2.60-2.65 and the value of See PDF for Equation is 4.0 x 10(-5). Some chemical c...

Effect of gonadotrophin-releasing hormone aned human chorionic gonadotrophin on the response of the ewe to pregnant mare serum gonadotrophin.

An experiment was conducted to determine whether gonadotrophin-releasing hormone (Gn-RH) or HCG would reduce the proportion of unruptured follicles in the Galway ewe following a high superovulating dose of PMSG given in conjunction with a progestagen to synchronize oestrus. Forty-four Galway ewes aged 1\m=1/2\to 5 years (average wt 64\m=.\7kg) were treated with intravaginal pessaries impregnate...

Pregnant Mare Serum Gonadotropin

Procedures have been developed for the purification of pregnant mare serum gonadotropin (PMSG) and its a and jl subunits. The procedure for the hormone purification involves three steps of column chromatography on Sephadex G-100, DEAE-Sephadex A-50, and hydroxyapatite. The preparation of subunits involves the dissociation of PMSG with 10 M urea followed by their separation by chromatography on ...

The Sensitivity of Ovarian and Oestrous Activity in Southdown Ewes to an Intramuscular Injection of Pregnant Mare Serum Gonadotrophin

A group of Southdown ewes (n=lll), two years and older, which had been maintained in isolation from rams had intravaginal sponges (Repromap, Upjohn) inserted on 18 Dee (day 0). On day 10 after sponge insertion rams (10%) were introduced to the ewes and at sponge withdrawal on day 12 they were harnessed and the ewes were allocated at random to PMSG treatment. The doses of PMSG were 0, 100, 200, ...

Chemical Studies on the Gonadotropic Hormone of Pregnant Mare Serum